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The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)
Das N-terminale 1-29-Fragment des humanen GHRH, derselbe aktive Kern wie bei Sermorelin, trgt vier stabilisierende Substitutionen (Position 2 D-Ala, 8 Gln, 15 Ala, 27 Leu), die fr den tetrasubstituierten Kern in Jett 2005 dokumentiert sind
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It will require well planned studies that account for weight and glycemic changes in order to firmly distinguish these pathways
