The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)
Bmj 374, 2103
12.5 mg of tirzepatide equals 62.5 units at a concentration of 20 mg/mL, or 50 units at a concentration of 25 mg/mL
Which concentration to choose for a 5 mg vial If you are following a standard dosing protocol starting at 2.5 mg per week, the 5 mg/mL concentration (1.0 mL water) is the most practical
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