In addition, subcellular structures such as mitochondria, vacuoles, and endoplasmic reticulum as well as reactive oxygen species (ROS) accumulation were observed under a fluorescence microscopy after stained with fluorescent dyes such as 27-DCF diacetate, Mito Tracker Green FM, Vacuole Membrane Marker MDY-64, and ER-Tracker Red dye
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These observations are strictly preclinical and are used to explore how circadian gene regulation intersects with tumor biology and treatment-response research
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It is thought that as copper is a redox-active transition metal and can participate in single electron reactions and catalyse the formation of free radicals, including undesirable hydroxyl radicals, it could contribute to oxidative stress characteristic of preeclampsia [93]
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