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Cell culture and treatments The intestinal endocrine L cell line STC-1 and islet cell line NIT-1 were cultured in RPMI 1640 medium supplemented with 5 mM glucose, 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin in 100 mm cell culture dishes at 37 C in a 5% CO 2 atmosphere

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It is a tetrameric enzyme consisting of four identical tetrahedrally arranged subunits of 60 kDa, which contains a single ferriprotoporphyrin group per subunit and has a molecular mass of about 240 kDa 2.1.3

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279, 5380653817.10.1074/jbc.M407676200 148 HolstB.SchwartzT

Note that symptoms can appear shortly after exposure or up to several days later