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First, an insulin-secreting -cell line MIN-6 cells stably expressing GLP-1R (GLP-1R-MIN-6 cells) was prepared, as we intended to observe a stronger effect of GLP-1R activation than that of endogenous GLP-1R

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In both groups before and after treatment, insulin peaked at 30 minutes but was prolonged and still present at 60 minutes after exenatide treatment ( Figure 4A )