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Briefly, the assay consisted of separately sequencing the 5UTR and the core amplicons obtained by Versant HCV genotype 2.0 using the BigDye Terminator v3.1 cycle sequencing kit and ABI 3500 Genetic Analyzer (Thermo Fisher Scientific-Applied Biosystems, Foster City, CA, USA) with a set of four primers, the 5UTR forward primer 5-GCAACAGGGAAYYTDCCUGGTTGCTC-3, 5UTR reverse primer 5-CTATCAGGCAGTACCACAAGG-3, core forward primer 5-GTGCCCCGGGAGGTCTCGTAG-3 and core reverse primer 5-CCAAGGGTACCCGGGCTG-3

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10.1007/BF02172145 45

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