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Hansen et al [17] used Hanks Balanced Salt Solution (HBSS) containing 5% FCS and 2 mM EDTA for both, while Cervantes-Barragan et al [15] replaced the HBSS with PBS and Luckashenak et al [16] used PBS for suspending the cells and HBSS for diluting the OptiPrep (both containing 0.2% BSA and 2 mM EDTA

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