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2.18 Multiplex enzyme-linked immunosorbent assay profiling of TCM and NCM To assess angiogenic, vascular injury, pro-inflammatory, cytokine and chemokine secretions a 54-plex ELISA kit separated across 7 plates was used (Meso Scale Discovery, Rockville, MD, USA)

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Exercise, as an important non-pharmacological intervention for disease prevention and rehabilitation, can regulate the level of mitophagy and can intervene in the pathological process of metabolic diseases by regulating mitophagy to inhibit OS, apoptosis, and inflammatory response