Confocal laser scanning microscopy (CLSM) analysis Seeds of two transgenic lines expressing redox-sensitive GFP2 (roGFP2) either in the cytosol (Grx1-roGFP2) or the plastids (TKTP-Grx1-roGFP2) 92 and Col-0 WT seedlings were sown onto 6-well cell culture plates (Sigma-Aldrich, CLS3516) containing 3 ml of liquid MS medium (pH 5.7) supplemented with 2% sucrose
Results and Discussion Engineering and Validation of the miniGrx1-roGFP2 Biosensor In our hands, the original Grx1-roGFP2 biosensor displayed somewhat inconsistent performance when fused to various targeting sequences for subcellular localization
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Screening vast collections of germplasm may reveal greater diversity and thereby favour the applicability of breeding strategies 25,752,757,758
Improved mental clarity Higher levels of glutathione have been linked to improved mental clarity and cognitive function
The low oxygen tension that characterizes this tissue may be an important component of the self-defense mechanism from free radical-mediated damage during spermatogenesis and Leydig cell steroidogenesis (3)
