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31 The lower H 2 O 2 levels in SHR in this study seems to suggest that H 2 O 2 might not have a significant direct role in the pathogenesis of hypertension in this strain of rat as despite the lower H 2 O 2 levels, systolic blood pressure still increased in the SHR

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This was followed by vortex mixing for ~1 min before centrifuging samples at 10,000 relative centrifugal force (RCF) for 20 min at 4C