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Agilent Technologies, USA) were coated with collagen IV and fibronectin and the cell-free background was measured in culture medium for each well by an xCELLigence RTCA SP device (Agilent Technologies)

The long-term impact on immune cell function and inflammatory microenvironment remains to be fully elucidated (65)

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While possible, maintaining results after stopping GLP-1 therapy is challenging and requires intensive lifestyle modifications including 200-300 minutes of weekly exercise, consistent dietary changes, regular self-monitoring, and often ongoing behavioral support

A fluorescence detector records the chromatograms

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