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The samples were run with a Bio-Rad CFX96 Real-time PCR system with a reverse transcription reaction for 10 min at 50 C, polymerase activation and DNA denaturation for 1 min at 95 C followed by 39 cycles of amplification (denaturation for 10 s at 95 C, annealing/extension for 15 s at 60 C) and a melt-curve analysis at 6595 C in 0.5 C increments
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