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PCR conditions were as following: initial denaturation at 95 C for 5 min, followed by 37 cycles of denaturation (94 C for 40 s), annealing (57 C for 30 s) and extension (72 C for 40 s), with the final extension at 72 C for 10 min

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Researchers should minimize repeated freeze-thaw cycles whenever possible to help preserve peptide stability and maintain experimental consistency

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Inflammatory mediators and blood brain barrier disruption in fatal brain edema of diabetic ketoacidosis