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Excess glutamate secreted from astrocytes drives upregulation of P-glycoprotein in endothelial cells in amyotrophic lateral sclerosis

Screening for DEGs in microarray data The R software package, limma (v3.52.4) 18 , was used to conduct differential gene analysis on two PD microarray datasets (GSE49036, GSE20164)

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The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

doi:10.1016/j.reprotox.2012.04.011