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Approximately 90% of the drug is rapidly inactivated by plasma pseudocholinesterase , while only about 10% reaches the neuromuscular junction (NMJ)

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In addition to its direct metabolism, arginine can be regenerated via the urea cycle through argininosuccinate synthase 1 (ASS1) and argininosuccinate lyase (ASL)

Some of the direct demonstrations of paracellular permeability include autoradiography with 3 H polyethylene glycol (PEG) 400 (Da), which reported the presence of the marker molecule in the intercellular spaces, in the enterocytes, and interestingly, in a subset of goblet cells, foreshadowing the discovery of GAPs [26]