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Controlled Disposal Dispose of unused material, solutions, and contaminated supplies under applicable laboratory rules

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Briefly, adherent INS-1E cells expressing the endogenous 2A -adrenergic receptors or transfected HEK-293 cells overexpressing human 2A -adrenergic receptor were dissociated from plates, and intact cells collected by centrifugation (600900 g, 510 min, 4 C)

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Data analysis was performed through MetaboAnalyst 6.0 platform ( accessed on 26 May 2024)