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Proteins were detected using the following antibodies: Lyn PerCP (clone C13F9, Cell Signaling Technology), SYK PE (clone B435666, Biolegend), PLC2 AF488 (clone K86-1161, BD Biosciences), pan AKT APC (Miltenyi Biotec), and S6 AF700 (clone 54D2, Cell Signaling Technologies)

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mTOR signaling disruption and its association with the development of autism spectrum disorder

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Use immediately after reconstitution or refrigerate at 28C within 15 minutes of mixing to preserve research-grade stability