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For all RNAi experiments, the L4440 plasmid lacking a gene of interest (empty RNAi) served as a control

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Flies were homogenized for 15 s using a Teflon micropestle and the homogenate centrifuged at 14,000 g for 2 min

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Data from 25 healthy volunteers displayed that free fatty acid metabolism is more sensitive to insulin than glucose metabolism, suggesting cooperation between glucose and free fatty acids in energy homeostasis, with free fatty acids playing the role of a buffer to support glucose stability [57]

Thyroglobulin (+ Antibodies to TPO and TG) When thyroglobulin (Tgbn) is higher in the context of an otherwise normally functioning thyroid, its likely the thyroid is laboring to overcome an iodine deficiency