Dreaming Up a Riddle for a Know-It-All"
For the detachment of adherent cells, TrypLE Express Enzyme (1) with Phenol Red (Gibco 12605010) was used
After removing detached cells by PBS washing, wounded cells were treated with 100 L of medium containing one of the following experimental conditions: SG (CTR), HG, HG supplemented with Epitalon at different concentrations (20, 40, or 60 ng/mL) or mannitol
Aqua, SLES, CAPB, Guar Hydroxypropyltrimonium Chloride, Carbomer, Dimethicone, EGMS, Sodium Chloride, EDTA, Perfume, Vitamin B5, 7 Herbs (Amla, Brahmi, Arnika, Aloe Vera, Wheat Germ Oil, Olive Oil, Coconut Oil), Vitamin E
As such, it is possible that redox-associated extrahepatic mechanisms may contribute to the activation of the hepatic AMPK pathway
Pavak K Shah, Silvia Gabriela HerreraLoeza, Christopher E Sims, Jen Jen Yeh, Nancy L Allbritton, "Small sample sorting of primary adherent cells by automated micropallet imaging and release", Cytometry Part A 85 (7): (2014)