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The quantification of plasma aromatic amino acid metabolites, PAA, PAGly, PAGln, and TMAO related metabolites was conducted by combining 10 l of plasma with 40 l of cold methanol containing individual isotope-labeled internal standard mix, followed by ultracentrifugation and LC/MS/MS analysis, following established protocols 108

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doi: 10.1038/nrc.2016.71 91 NeermannJWagnerR

Giacometti et al., 2013