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After incubation with hypo-osmotic swelling solution [HOS, containing 6.75 g/L fructose and 3.67 g/L sodium citrate] at 37C for 1 h, plasma membrane integrity of each group (repeated in triplicated)was analyzed by evaluating the modifications of sperm tail to score swollen sperm according to previous publications (61)

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doi: 10.1007/s12011-010-8625-1 126 MatroneAFerrariFSantiniFEliseiR

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