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15 Although the primary function of GLP-1 is to serve as an anorexigenic (appetite-reducing) and incretin hormone, as well as a mediator of the ileal brake through inhibition of upper gastrointestinal function to prevent malabsorption and postprandial metabolic disturbances, the influence of GLP-1 on colon function was evaluated by a limited number of studies

Intracellular GSH/GSSG measurement The GSH and GSSG levels in whole liver tissues or cultured cells were measured using OxiSelect Total Glutathione (GSSG/GSH) Assay Kits in accordance with the manufacturers instructions (Cell Biolabs, San Diego, CA, USA)