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Menomonee Falls, Wisconsin: Record Research, Inc

Science 351, 933939

Samples were then treated with RNase A (Roche) and Proteinase K (Roche), and the DNA was purified using the QIAquick PCR Purification kit (QIAGEN, 28106) according to the manufacturer's instructions

Discussion While it is well accepted that EtOH-associated oxidative stress is a key mediator in the pathogenesis of ALD, the molecular details governing this process remain incompletely understood