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Severe allergic reactions to local anaesthetics are uncommon

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It helps in identifying the presence and the overgrowth type

Yes, as long as it has not been exposed to excessive heat or freezing temperatures

Fast Blue is typically found in the cell body and proximal dendrites of the labeled neurons while the nuclei always remain unstained (see insertion in Figure 1A)

In fact, a difference in experimental conditions as well as cell-type specificity of plasticity mechanisms and heterogeneity of recorded subpopulations of interneurons could have contributed to discrepancies between the findings of the above studies, e.g., whether CA1 inhibitory neurons express predominantly LTD (McMahon and Kauer, 1997) or also LTP (Taube and Schwartzkroin, 1987