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Ghigo E, Arvat E, Muccioli G, Camanni F

Tumor cell transplantation MC38 (Kerafast, Boston, MA) and E0771 (CRL-3461, ATCC, Manassas, VA) cells were maintained at 37 C with 5% CO 2 in Dulbecos Modified Eagle Medium containing 4.5 g/L glucose (Gibco, Carlsbad, CA) and supplemented with 10 mM HEPES buffer (Gibco), 10% fetal bovine serum (VWR International, Radnor, PA), 2 mM glutamine (Gibco), and 1% penicillin streptomycin (Gibco)

There are three primary areas approved for subcutaneous injections: The Abdomen: This includes the area above, below, and to the sides of the belly button, staying outside the two-inch radius

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To view a copy of this licence, visit About this article Cite this article Kong, F., Zhao, Y., Zhang, W