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The sample size was determined based on the following rationale: for system consistency validation, ninety-six parallel samples ( n = 96) were analyzed to comprehensively cover all detection channels of a single 96-well plate, a number that far exceeds conventional requirements for microbiological method validation and aims to provide highly stable mean and relative standard deviation (RSD) values

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[18] Ethanol Patch Test - Place an ethanol-soaked bandage directly on the skin for twenty minutes

PMID 15470076

However, it's generally understood that over time the perceptible benefits may diminish, requiring a period of discontinuation 1