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Low levels of serotonin have been linked to depression

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Transduction of spermidine and spermine co-transmitters Polyamines interact with a range of receptors and ion channels on the intracellular side of the cell membrane, conveying inward rectification in some cases and acting as modulators in others (Moriyama et al., 2020)

Angle: 45 to the skin surface (or 90 if pinching a larger fat fold)