The primary antibodies were rabbit anti-FGF5 antibody (Thermo Fisher Scientific, USA), goat anti-SRD5A1 antibody (Abcam, UK), rabbit anti-androgen receptor antibody (Abcam, UK), goat anti-DKK1 antibody (Abcam, UK), beta-catenin polyclonal antibody (Proteintech, USA), c-MYC polyclonal antibody (Proteintech, USA), and beta tubulin monoclonal antibody (Proteintech, USA)
Cells were washed again three times with DPBS and blocked for 1 h with 1% BSA (Fisher Scientific, BP1600-1) in TBST at 4 C
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