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Membranes were washed three times in 1PBS and positive signals were developed with enhanced chemiluminescence kit (Amershan Pharmacia Biotech, Little Chalfont Buckinghamshire, England)

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Systemic injection of LPS induces region-specific neuroinflammation and mitochondrial dysfunction in normal mouse brain

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In the case of muscle, for instance, IGF1-LR3 does not cause muscle cells to get larger, but it does increase total numbers of muscle cells