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All embryos were cultured using sequential culture medium (Vitrolife, Gothenburg, Sweden) in incubators at 37C under 5% O 2 , 6% CO 2 , and 89% N 2 conditions with high moisture until day 6

GLP-1 agonists commonly cause mild gastrointestinal effects like nausea or reduced appetite, which often subside within weeks

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By stimulating the pituitary rather than flooding the bloodstream with hormone, this approach is designed to preserve that pulsatile pattern