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Intriguingly, an association between the prevalence of severe tetracycline-treated acne and increased risk of prostate cancer later in life has been observed [20]

Neurochem Res 45 , 14611480 (2020)

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After that, cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA, Abcam) at RT for 1 h