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Additionally, a potential drug binding site can also be predicted based on previous mutagenesis studies that allowed the identification of residues implicated in the transport of doxorubicin, vincristine, and etoposide

This creates docking sites for IRS-1 (insulin receptor substrate 1), which then recruits PI3K through its p85 regulatory subunit

Its cardiotoxicity-diminishing activity seems to be due to the removal of iron from ironanthracycline complexes, and thus it may reduce free radical formation

however, mutations associated with NDDs were mostly rare