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LEfSe is known to reduce false positives and can highlight biological consistency across subclasses, while the KruskalWallis test used alone can achieve a lower false-negative rate compared to LEfSe 38

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Targeted MRM-based methods are most easily developed with the availability of purified proteins, whereas untargeted DDA analyses offer a more flexible, universal application without time-consuming optimization steps

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While compounded medications are not FDA-approved, 503A pharmacies maintain rigorous quality control and sterility protocols