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MCs were lysed in 400 L of 10% DMSO in 1 N NaOH (extraction buffer) and incubated with agitation (24 h at 100 C for pMCs and 18 h at 80 C for iMCs)

Cellular Mineral Transport The copper-binding component delivers bioavailable copper to research systems, activating internal enzymatic antioxidant models

In addition, these agents also limit lipid peroxidation, oxidative protein and nucleic acid modifications, and thus preserve mitochondrial integrity and maintain ovarian steroidogenic function [265]