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We selected pAcF as the handles for constructing conjugates instead of other chemistry such as azide mediated click chemistry as the cyclopropane-tetrazine ligation employed in the pAcF system demonstrates superior bioorthogonality compared to azide-alkyne cycloadditions, effectively minimizing off-target reactions in complex cellular environments

10.1002/glia.24531 32 BavaR.CastagnaF.MusellaV.LupiaC.PalmaE.BrittiD

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