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The difference in results is dramatic, and it comes down to one thing: understanding how these two approaches interact at a biological level

To evaluate the functional relevance of the GLP-1R cholesterol binding sites identified during the cgMD simulations, 12 residues were selected within or at the periphery of these sites and mutated to alanine by site-directed mutagenesis (Figure 2F)

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Metab., 2006