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The samples were incubated at 20 C for 12 h, then gradually warmed to 4 C over a 4-h period

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Short-term effects of oral corticosteroids: Loss of energy Increased risk of infection Increased thirst and appetite Weight gain Vomiting Diarrhea Panting Frequent urination Long-term effects of oral corticosteroids: Lethargy/fatigue (no energy to play) Change in behaviour (such as aggression) Suppressed immune system function Increased risk of bacterial/fungal infections Increased risk of urinary tract infections Poor wound healing Stomach ulcers Diabetes Weight gain / obesity Impaired wound healing Cushing's disease Loss of bladder control Hair loss Increased blood pressure Allergic reaction Skin or coat changes 5 These side effects may reduce the quality of life of not only the affected cat, but also the family relationship

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