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developed an acid hydrolysis method for the quantification of free and total carnitine in plasma samples by electrospray ionization tandem mass spectrometry (ESI-MS/MS) (Johnson, 2010)

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Results GSDMD-mediated pyroptosis is activated in ischemic hindlimbs To investigate the involvement of GSDMD-mediated pyroptosis in hindlimb ischemic injury in vivo, we established hindlimb ischemia (HLI) model by ligation of the femoral artery in wild-type (WT) mice