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After dispensing into 96-well white plates, a baseline read of luminescent signal was serially recorded over 5 min using a Flexstation 3 instrument (Molecular Devices) at 37C before addition of the indicated concentration of ligand, after which signal was repeatedly recorded for 30 min

For more information: Karen Elkind-Hirsch, MSc, PhD, HCLD , can be reached at [email protected].

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