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Processing of tissue samples and hemoglobin estimation Blood, 100 L of sample drawn by cardiac puncture under ether anesthesia (50 g/kg body weight) are centrifuged and washed twice with 5 mL of 0,9% NaCl

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Funding This study was supported by Lombardy region as part of the EPIFARM-Pharmaco-epidemiology Agreement with the Istituto di Ricerche Farmacologiche Mario Negri IRCCS