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The kit can also be used to measure only GSSG by following an alternative protocol

Under physiological conditions, more than 98% of the total GSH pool exists in the reduced form, thereby shaping cellular redox potential and influencing proliferation, differentiation, and cell death 5 .Although the GSH/GSSG ratio has long been regarded as an indicator of cellular redox status, recent live-cell studies using genetically encoded redox sensors indicate that increases in GSSG are typically transient and compartmentalized rather than sustained intracellular accumulations 6,7

Quantitative RT-PCR RNA was isolated using a NucleoSpin kit (Machery-Nagel, 740955.50) and converted in cDNA using an iScript cDNA Synthesis Kit (Biorad)

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