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Diluent Solution 1(Acetonitrile : HPLC Grade Water:: 50:50) Take 500 ml of Acetonitrile and 500 ml of HPLC grade water by using measuring cylinder, transfer into reagent bottle, mix well and degas in sonicator and keep at room temperature

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Animals Four-week-old male SD rats of specific pathogen-free (SPF) grade for experiments were obtained from Nanjing Qinglong-shan Animal Farm (SCXK(SU) 2017-0001, China)

PMID: 265572