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Based on 1 H NMR, the grafting ratio of alanine and GSH were 0.027 and 0.13, respectively, calculated by the ratio of the integrated areas of proton f on alanine and proton l on GSH (Fig

El sobrenadante resultante fue evaporado bajo gas nitrgeno a 45C hasta secarse y se adicionaron 50 mL de HCL butanlico 1 mol/L a cada muestra (cada pozo) e incubados a 60C durante 15 min (derivatizacin)