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In liver mitochondria, cholesterol accumulation has been found to disrupt mitochondrial functional performance which can contribute to oxidative stress and organ injury 60

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When this system gets disruptedby gut issues, inflammation, or insulin resistanceit can cause problems with hunger control, fat storage, and blood sugar regulation

Scandinavian J Gastroenterology

after 6 h of transfection, the medium was replaced with fresh medium for continuous culture