To combine Snap25 detection with GLP-1 immunostaining, chromogenic RNAScope was performed first, followed by an immunostaining for GLP-1 exactly as described before (see also Table 1)
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Potassium, magnesium, and calcium each play specific roles in the cardiac action potential, the electrical event that triggers each heartbeat
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However, both visceral and subcutaneous fat play significant but differing roles in systemic inflammation 101 , 102 and it is therefore possible that the improved outcomes are the result of improvements in obesity and T2DM rather than a direct effect of the GLP-1RA