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White spots or discoloration post-injection can indicate blanching, caused by interrupted blood supply to the skin

For routine reconstitution with repeated withdrawal, bacteriostatic water is more suitable thanks to its extended shelf life

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Optogenetic experiments After AAVs infection and to deliver blue light stimulation, a zirconia ferrule of 1.25 mm diameter with multimode optical fiber (200 m, Thorlabs) was implanted in the LH (from bregma (mm): AP -1.3, ML 1.0, and from dura (mm): DV -5.3)

Instrumentation Calibration: Flow meters, pressure sensors, TOC analyzers, and other monitoring instruments must be calibrated at regular intervals to ensure accurate readings