Cells were treated with 1 mL 0.25% trypsin in the incubator and, after 5 min, RPMI 1640 (with 10% FCS, 1% Hepes, 1% Penstraps, 1.1% glucose) was added to block the trypsin activity
There is usually a high expression level of CK and HMB-45 in the large pigmented epithelioid cells, but a low expression level of S-100 protein
1: International Stem Cell Initiative Consortium, Akopian V, Andrews PW, Beil S, Benvenisty N, Brehm J, Christie M, Ford A, Fox V, Gokhale PJ, Healy L, Holm F, Hovatta O, Knowles BB, Ludwig TE, McKay RD, Miyazaki T, Nakatsuji N, Oh SK, Pera MF, Rossant J, Stacey GN, Suemori H
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Regarding the opposite condition, i.e., optimal iodine supply, it has been presented in a recently published review that nutritional interventions, including iodine supplementation, are able to ameliorate the effect of endocrine disruptors on human reproductive health [60]
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