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Every time you insert a needle into a vial, there is a small risk of introducing contaminants, even with proper technique

doi: 10.1046/j.1528-1157.2001.17601.x

(A) 6th May 2024

70 LiH.XuH.LiY.JiangY.HuY.LiuT.et al (2020)

The organic layers from two extractions were combined and aliquots were analyzed on a 4000 Q-Trap mass spectrometer (AB SCIEX, Framingham, MA) using the 50 2.1 mm Hydrophilic Interaction Liquid Chromatography (HILIC-LC) column (Waters Corp., Milford, MA)

For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35