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Refluxed blood promotes the Fenton reaction, terminating in the production of hydroxyl radical, the most potently destructive ROS

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Briefly, proteins were subjected to sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (SDS-10% PAGE) under reducing conditions in Laemmlis buffer system followed by transfer to nitrocellulose membranes 19

Studies show that supplementation with L-Carnitine can increase the amount of fat burned during exercise by up to 30%