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Oxytocin Differentiated effects according to the administration route in a prenatal valproic acid-induced rat model of autism

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This is also referred to as IV Glutathione therapy

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Loading 1 mL DEAE-Sephadex A-25 (Sigma-Aldrich) into chromatographic column and cover with some quartz sand, add the extracted GSL sample into column then wash the column successively by 70% MeOH, ddH 2 O and 20 mM acetate solution, incubated the sample overnight at RT after adding 0.5 mL sulfatase (Sigma-Aldrich), collect GSL extraction by 1.5 mL ddH 2 O washing for further analysis